EDUCATION

1991-1996                Bs Biology. Universidad de Chile.

1997-2002                PhD, Ecology, Pontificia  Universidad Católica de Chile. Evolutionary Quantitative Genetics of Bioenergetics in a Wild Mammal Population

2001-2002                Postdoctoral Stay in Center for Advances Studies in Ecology and Biodiversity (CASEB), program 1. Department of Ecology, P. Universidad Católica de Chile.

 

APPOINTMENTS (2015-2022)

2008-2010                  Director, Institute of Ecology and Evolution, Austral University of Chile.

2007-2009                  Coordinator (head) of Doctorate Program in Systematics and Ecology, Austral University of Chile.

2007-2009                  Member of the Fondecyt (national funding agency for basic sciences) evaluating committee of Biology 1 (Ecological, Genetic and Evolutionary Sciences).

2008-2010                  Member of “Becas Chile” (national funding agency for graduate fewllowships) evaluating committee of Biology 1 (Ecological, Genetic and Evolutionary Sciences).

2001- 2012                 Director, Doctorate Program in Ecology & Evolution

2014-2016                  Director, Magister in science with mention Genetics

2003- on going          Associate professor, Institute of Ecology and Evolution, Austral University    of Chile.

POSTDOCTORAL SUPERVISION

Paulina Artacho (funded by FONDECYT)

Marcela Franco (funded by FONDECYT)

Luis Castañeda (funded by FONDECYT)

Ph.D SUPERVISION

2008     Paulina Artacho  (PhD in Systematics and Ecology, UACH)

2009     Luis Castañeda  (PhD in Systematics and Ecology, UACH)

2012      Juan Diego Gaitan  (PhD in Systematics and Ecology, UACH)

2011     Rodrigo Scheihing  (PhD in Systematics and Ecology, UACH)

2013      José Luis Bartheld    (PhD in Systematics and Ecology, UACH)

2014     Pablo Cortés  (PhD in Ecology and Evolution, UACH)

2020      Jaiber Solano Iguaran (PhD in Ecology and Evolution, UACH)

2021      Julian F. Quintero Galvis (PhD in Ecology and Evolution, UACH)

MASTER SUPERVISION

2016      Julian F. Quintero Galvis (Msc science mention Genetics, UACH)

2017     Pedro Ratamal (Msc science mention Ecology, UACH)

2018     Rocio Paleo (Msc science mention Genetics, UACH)

2022     Carlos Mejías (Msc science mention Ecology, UACH)

UNDERGRADUATE THESIS DIRECTION

2010     Pablo Cortés (Bs. Biology, UACH)

2012     Andrea Bruning (Bs. Biology, UACH)

2012     Carolina Contreras  (Bs. Biology, UACH)

2017      Julio Figueroa (Bs. Biology, UACH)

2019     Pablo Quintrel (Ing. Biotechnology, USACH)

2020    Sebastian Flores  (Ing. Biotechnology, USACH)

2020    Rodrigo Contreras (Veterinarian, UACH)

2021    Paulina Gutiérrez  (Natural Resources Conservation Engineer, UACH)

2021    Macarena Alvarado (Veterinarian, UACH)

2022    Claudia Pereira  (Veterinarian, UACH)

2022     Isabella Peña Rocco (Veterinarian, UACH)

2022    Maite Villalobos Avedaño (Veterinarian, UACH)

Current Tamara Estay  (Bs. Biology, UPC)

Current Felipe Martín Ortiz Orellana (Veterinarian, UACH)

Current Francesca Nicole Cáceres (Bs. Biology, UACH)

Current María Isidora Camus Rojas (Bs. Biology, UCHILE)

HONORS AND AWARDS (2015-2022)

2004     Selected for the Chilean Academy of Sciences, as a part of the “Ciencia de Frontera” group. A small division of the Academy composed by young and productive scientists from all disciplines.

2010     George Bartholomew Award. Prize for young researchers from the Society for Integrative and Comparative Biology (www.sicb.org).

2010:     Sciences Faculty, Universidad Austral de Chile. First Young Investigator Award.

GRANTS and funding

2007-2009 PSD-89:

“Inserción de Investigadores en la Academia” Funding for hiring three new researchers at Institute of Ecology and Evolution. Permanent hiring was compromised by the University at the end of the grant (U$ 250.000).

 

2008-2010. MECESUP:

Program for reinforcement of the graduate program (funds for fellowships and travel for students, U$ 450.000).

 

2008-2011. FONDECYT (regular program):

Invasiones Biologicas: Evolucion Adaptativa, Historia Y Manejo Ambiental De Una Plaga Exotica(U$ 280.000). Co-investigator.

 

2009-2011. ECOS-CONICYT (academic interchange program with France):

The effects of sexual reproduction on the genetic architecture of life history traits using aphids as biological models. (U$ 60.000).

 

2009-2012. FONDECYT 1090423:

Natural selection, quantitative genetics and evolutionary physiology: lessons from a cosmopolitan species, Helix aspersa. (U$ 290.000).

 

2013-2016. FONDECYT 1130750:

“Testing the role of trade-offs for long term evolution: an evolutionary experiment using microbes”.

 

2016-2019 FONDECYT 1160115.

Passerine birds facing marine environments: feeding on salty prey and fighting with oxidative stress. Co-investigador

 

2018-2021 FONDECYT 1180917

Studying the biology of a relict mammal, Dromiciops gliroidesusing landscape genomics and isotopic ecology

 

2022-2025 FONDECYT 1221073

Studying the adaptive significance of hibernation and cold adaptation in marsupials: lessons from the microbiotherid marsupial monito del monte (genus Dromiciops)

 

2021-2024 NUCLEO LILI

Millenium Nucleus of Patagonian Limit-of-Life 2021

 

PUBLICATIONS 

134          MOLINET J., EIZAGUIRRE J., QUINTREL P., BELLORA N., VILLARREAL, P., NESPOLO, R.F.,CUBILLOS, F.A., (2022).Natural Variation in Diauxic Shift between Patagonian Saccharomyces eubayanus Strains. Msystems. 7. 6. e00640-22. https://doi.org/10.1128/msystems.00640-22

133          NESPOLO, R.F.,PEÑA I., MEJIAS, C., ÑUNQUE A., ALTAMARIANO T., & BOZINOVIC, F. (2022).Communal nesting is the optimal strategy for heat conservation in a social marsupial: lessons from biophysical models.Journal of Experimental Biology. 225. 22. jeb244606. https://doi.org/10.1242/jeb.244606

132          NESPOLO, R.F.,MEJIAS, C., & BOZINOVIC, F. (2022).Isometric scaling of hibernation: when trees do not let the forest be seen.Proceedings of the Royal Society B. 289. 1984. https://doi.org/10.1098/rspb.2022.1719

131          FENG S., BAI M., RIVAS-GONZALES I., LI C., FRANCO L.,  GAITÁN-ESPITIA J.D., NESPOLO, R.F. WARREN J., SCHIERUP M., ZHANG G.  (2022). Incomplete lineage sorting and phenotypic evolution in marsupials. Cell. 185, 10. 1646-1660. https://doi.org/10.1016/j.cell.2022.03.034

130          SAENZ-AGUDELO, P., PELUSO L., NESPOLO, R.F. BROITMAN B., HAYE P., LARDIES M.(2022).Population genomic analyses reveal hybridization and marked differences in genetic structure of Scurria limpet sister species with parapatric distributions across the South Eastern Pacific. Ecology and Evolution. 12, 5. e8888. https://doi.org/10.1002/ece3.8888

129          MEJIAS, C., NAVEDO J.G., SABAT P., FRANCO L.,  BOZINOVIC, F. & NESPOLO, R.F. (2022). Body Composition and Energy Savings by Hibernation: Lessons from the South American Marsupial Dromiciops gliroides.  Physiological and Biochemical Zoology. 95, 3. https://doi.org/10.1086/719932

128          NESPOLO, R.F.,MEJIAS, C., & BOZINOVIC, F. (2022). Why bears hibernate? Redefining the scaling energetics of hibernation.Proceedings of the Royal Society B. 289. 1973. https://doi.org/10.1098/rspb.2022.0456

127          MOLINET J., URBINA K., VILLEGAS C., ABARCA V., OPORTO C., VILLARREAL, P., SALINA F., NESPOLO, R.F.,CUBILLOS, F.A., (2022).A Saccharomyces eubayanus haploid resource for research studies. Scientific reports. 5976 (2022). https://doi.org/10.1038/s41598-022-10048-8

126          VILLARREAL, P., VILLARROEL, C.A., O’DONNELL, S., AGIER, N., QUINTERO?GALVIS, J.F., PEÑA, T.A., NESPOLO, R.F.,FISCHER, G., VARELA, C., CUBILLOS, F.A., (2022). Late Pleistocene?dated divergence between South Hemisphere populations of the non?conventional yeast L . cidri. Environmental Microbiology. 1–21. https://doi.org/10.1111/1462-2920.16103

125          QUINTERO-GALVIS, J. F., SAENZ-AGUDELO, P., AMICO, G. C., VAZQUEZ, S., SHAFER, A. B. A., & NESPOLO, R. F.(2022).Genomic diversity and Demographic History of the Dromiciops genus (Marsupialia: Microbiotheriidae).Molecular Phylogenetics and Evolution, 168. 107405. doi.org/10.1016/j.ympev.2022.107405

124          FONTÚRBEL, F., FRANCO M, BOZINOVIC, F. QUINTERO?GALVIS, J. F., MEJIAS C, AMICO G, VASQUEZ S, SABAT P, SANCHEZ-HERNANDEZ J,WATSON D, SAENZ-AGUDELO, P., & NESPOLO RF. (2022).The ecology and evolution of the Monito del monte, a relict species from the southern South America temperate forests. Ecology and Evolution. 10.22541/au.163782997.73725373/v1

123          NESPOLO RF, FONTÚRBEL, F., MEJIAS, C., CONTRERAS, R., GUTIERREZ, P. ODA E, SABAT P, HAMBLY C, SPEKMAN JR, BOZINOVIC, F. (2022). A Mesocosm Experiment in Ecological Physiology: The Modulation of Energy Budget in a Hibernating Marsupial under Chronic Caloric Restriction. Physiological and Biochemical Zoology.95. 1. /doi.org/10.1086/717760

122          VILLARREAL, P, QUINTREL PA, OLIVARES-MUÑOZ, RUIZ J, NESPOLO RF & CUBILLOS, F. A. (2022). Identification of new ethanol?tolerant yeast strains with fermentation potential from central Patagonia. Yeast. doi.org/10.1002/yea.3662

121          NAVARRETE L, BOZINOVIC, F. PEÑA-VILLALOBOS I, CONTRERAS C, SANCHEZ-HERNANDEZ J, NEWSOME S.D, NESPOLO RF, SABAT P. (2021). Integrative Physiological Responses to Acute Dehydration in the Rufous-Collared Sparrow: Metabolic, Enzymatic, and Oxidative Traits. Frontiers in Ecology and Evolution. doi.org/10.3389/fevo.2021.767280

120          FONTÚRBEL, F., NESPOLO RF, AMICO G &WATSON D.M. (2021). Climate change can disrupt ecological interactions in mysterious ways: Using ecological generalists to forecast community-wide effects. Climate Change Ecology. 2. doi.org/10.1016/j.ecochg.2021.100044

119          MARDONES, W., VILLARROEL, C.A., ABARCA V, URBINA, K., PEÑA T, MOLINET J, NESPOLO RF & CUBILLOS, F. A. (2021).Rapid selection response to ethanol in Saccharomyces eubayanus emulates the domestication process under brewing conditions. Microbial Biotechnology, 1–18. https://doi.org/10.1111/1751-7915.13803

118          SABAT P, NEWSOME S, PINOCHET S, NESPOLO, R. F.,SANCHEZ-HERNANDEZ J, MADONADO K, GERSON A, SHARP Z, WHITEMAN JP. (2021). Triple Oxygen Isotope Measurements (?’17O) of Body Water Reflect Water Intake, Metabolism, and ?18O of Ingested Water in Passerines.Frontiers in Physiology. https://doi.org/10.3389/fphys.2021.710026

117          MEJIAS, C., CASTRO-PASTENE, C., CARRASCO, H., QUINTERO?GALVIS, J. F., SOTO-GAMBOA, M., BOZINOVIC, F., & NESPOLO, R. F.(2021).Natural history of the relict marsupial Monito del Monte at the most extreme altitudinal and latitudinal location. Ecosphere, 9639. doi:10.1002/ecs2.3577

116          NESPOLO, R. F.,MEJÍAS, C., ESPINOZA, A., QUINTERO-GALVIS, J. F., REZENDE, E. L., FONTÚRBEL, F. E., & BOZINOVIC, F. (2021). Heterothermy as the Norm, Homeothermy as the Exception: Variable Torpor Patterns in the South American Marsupial Monito del Monte (Dromiciops gliroides). Frontiers in Physiology. doi:10.3389/fphys.2021.682394.

115          QUINTERO-GALVIS, J. F., SAENZ-AGUDELO, P., CELIS-DIEZ, J. L., AMICO, G. C., VAZQUEZ, S., SHAFER, A. B. A., & NESPOLO, R. F.(2021). The biogeography of Dromiciops in southern South America: Middle Miocene transgressions, speciation and associations with Nothofagus.Molecular Phylogenetics and Evolution, 163, 107234. doi:10.1016/j.ympev.2021.107234

114          GIROUD S, K., HABOLD, C., NESPOLO, R. F.,MEJIAS C, TERRIEN, T., LOGAN S, HENNINING R & STORY K (2021). The torpid state: Recent advances in metabolic adaptations and protective mechanisms. Frontiers in Physiology. 11: 623665. doi: 10.3389/fphys.2020.623665

113          URBINA, K., VILLARREAL, P., NESPOLO, R. F.,SALAZAR, R., SANTANDER, R., & CUBILLOS, F. A. (2020). Volatile Compound Screening Using HS-SPME-GC/MS on Saccharomyces eubayanus Strains under Low-Temperature Pilsner Wort Fermentation. Microorganisms, 8(5), 755. doi:10.3390/microorganisms8050755

112          NESPOLO, R. F.,VILLARROEL, C. A., OPORTO, C. I., TAPIA, S. M., VEGA-MACAYA, F., URBINA, K., … CUBILLOS, F. A. (2020). An Out-of-Patagonia migration explains the worldwide diversity and distribution of Saccharomyces eubayanus lineages. PLoS Genetics, 16(5), e1008777. doi:10.1371/journal.pgen.1008777

111          NESPOLO RF, SOLANO?IGUARAN, J. J., PALEO?LÓPEZ, R., QUINTERO?GALVIS, J. F., CUBILLOS, F. A., & BOZINOVIC, F. (2020). Performance, genomic rearrangements, and signatures of adaptive evolution: Lessons from fermentative yeasts. Ecology and Evolution. ece3.6208. doi:10.1002/ece3.6208

110          MARDONES, W., VILLARROEL, C.A., KROGERUS, K., TAPIA, S.M., URBINA, K., OPORTO, C.I., NESPOLO RF & CUBILLOS, F. A.2020. Molecular profiling of beer wort fermentation diversity across natural Saccharomyces eubayanus isolates. Microbial Biotechnology, 13(4), 1012–1025. doi:10.1111/1751-7915.13545

109          REZENDE E.L, BACIGALUPE L.D, NESPOLO RF & BOZINOVIC F, (2020). Shrinking dinosaurs and the evolution of endothermy in birds. Science Advances, 6(1), eaaw4486. doi:10.1126/sciadv.aaw4486

108          ODA, E., RODRÍGUEZ-GÓMEZ, G.B., FONTÚRBEL, F.E., SOTO-GAMBOA, M. & NESPOLO, R.F. 2019. Southernmost records of Dromiciops gliroides: extending its distribution beyond the Valdivian rainforest. Gayana (Concepción)83: 145–149.

107          SABAT P, BOZINOVIC F, CONTRERAS-RAMOS C,NESPOLO RF.NEWSOME SH, QUIRICI V, MALDONADO K, PEÑA-VILLALOBOS I, RAMIREZ-OTAROLA N & SANCHEZ-HERNANDEZ JC. (2019). The interplay between ambient temperature and salt intake affects oxidative status and immune responses in a ubiquitous Neotropical passerine, the rufous-collared sparrow. Comparative Biochemistry and Physiology -Part A, 234, 50–59. doi:10.1016/j.cbpa.2019.04.016

106          D’ELIA, G, FABIAN J, LEONARDO B, BOZINOVIC F, CANTO J, CORREA CJ, FONTÚRBEL F , LISÓN F, MÉNDEZ M, NESPOLO RF, OPAZO J, PALMA E, RAU JA, RODRÍGUEZ S, RODRÍGUEZ-SERRANO E, SABAT P, VÁSQUEZ R, & VICTORIANO P. (2019). “Sugerencias Para Mejorar La Regulación Chilena de Manipulación de Vertebrados Terrestres En Poblaciones Naturales En El Contexto de Investigaciones Científicas.” Gayana 83(1):1–5.

105          RAMÍREZ-CÁCERES, G.E., MOYA-HERNANDEZ, M., QUILODRAN, M, NESPOLO, R.F.,CEBALLOS R., VILLAGRA, C.A & RAMÍREZ C.C.(2019). Harbouring the secondary endosymbiont Regiella insecticola increases predation risk and reproduction in the cereal aphid Sitobion avenae. Journal of Pest Science. 1–9.

104          TAPIA-MONSALVE, R., NEWSOME, S.D., SANCHEZ-HERNANDEZ, J., NESPOLO, R.F., BOZINOVIC F.  SABAT, P. (2018). Terrestrial birds in coastal environments: metabolic rate and oxidative status varies with the use of marine resources. Oecologia. 188: 65–73.

103          NESPOLO, R.F.,GAITAN-ESPITIA, J.D., QUINTERO-GALVIS, J.F., FERNANDEZ, F.V.,SILVA, A.X., MOLINA, C., STOREY K.B & BOZINOVIC F.  (2018). A functional transcriptomic analysis in the relict marsupial Dromiciops gliroidesreveals adaptive regulation of protective functions during hibernation. Molecular Ecology. 27: 4489–4500.

102          QUINTERO-GALVIS J.F.,PALEO-LOPEZ R., SOLANO-IGUARAN J.J., LEDGER T., POUPIN M.J., GAITÁN-ESPITIA J.D., ANTO? A., TRAVISANO M. & NESPOLO R.F.(2018).Exploring the evolution of multicellularity in Saccharomyces cerevisiaeunder bacteria environment: an experimental phylogenetics approach.  Ecology and Evolution. 8(9), 4619–4630.

101          NESPOLO R.F., GONZALEZ-LAGOS C., SOLANO-IGUARAN J.J.,ELFWING E., GARITANO-ZAVALA A., MAÑOSA S., ALFONSO J.C. & ALTIMIRAS J (2018).Aerobic power and flight capacity in birds: a phylogenetic test of the heart-size hypothesis.  The Journal of experimental biology.doi: 10.1242/jeb.162693

100          WIJENAYAKE, S., LUU, B.E., ZHANG, J., TESSIER, S.N. QUINTERO-GALVIS J.F, GAITÁN-ESPITIA J.D., NESPOLO R.F.& STOREY K.B. (2018).Strategies of biochemical adaptation for hibernation in a South American marsupial Dromiciops gliroides: 4. Regulation of pyruvate dehydrogenase complex and metabolic fuel selection.Comparative Biochemistry and Physiology Part – B: Biochemistry and Molecular Biology.224, 32-37.

99            LUU, B.E., WIJENAYAKE, S., ZHANG, J., TESSIER, S.N. QUINTERO-GALVIS J.F, GAITÁN-ESPITIA J.D., NESPOLO R.F.& STOREY K.B. (2018).Strategies of biochemical adaptation for hibernation in a South American marsupial Dromiciops gliroides: 3. Activation of pro-survival response pathways. Comparative Biochemistry and Physiology Part – B: Biochemistry and Molecular Biology.224, 26–31.

98            LUU, B.E., WIJENAYAKE, S., ZHANG, J., TESSIER, S.N. QUINTERO-GALVIS J.F,GAITÁN-ESPITIA J.D., NESPOLO R.F.& STOREY K.B. (2018).Strategies of biochemical adaptation for hibernation in a South American marsupial Dromiciops gliroides: 2. Control of the Akt pathway and protein translation machinery.Comparative Biochemistry and Physiology Part – B: Biochemistry and Molecular Biology.224, 19–25.

97            WIJENAYAKE, S., LUU, B.E., ZHANG, J., TESSIER, S.N. QUINTERO-GALVIS J.F,GAITÁN-ESPITIA J.D., NESPOLO R.F.& STOREY K.B. (2018).Strategies of biochemical adaptation for hibernation in a South American marsupial Dromiciops gliroides: 1. Mitogen-activated protein kinases and the cell stress response.Comparative Biochemistry and Physiology Part – B: Biochemistry and Molecular Biology.224, 12–18.

96            BACIGALUPE LD, MOORE A.J, NESPOLO R.F, REZENDE E &BOZINOVIC F. (2017). Quantitative Genetic Modeling of the Parental Care Hypothesis for the Evolution of Endothermy. Frontiers in Physiology. 8: 1005.

95            SABAT P, NARVÁEZ C, PEÑA-VILLALOBOS I, CONTRERAS C, MALDONADO K, SANCHEZZ-HERNANDEZ J.C, NEWSOME S.D, NESPOLO R.F &BOZINOVIC F. (2017). Coping with Salt Water Habitats: Metabolic and Oxidative Responses to Salt Intake in the Rufous-Collared Sparrow. Frontiers in Physiology. 8: 654.

94            VERGARA-AMADO J, SILVA A.X, MANZI C, NESPOLO R.F, &CARDENAS L. (2017). Differential expression of stress candidate genes for thermal tolerance in the sea urchin Loxechinus albus. Journal of Thermal Biology, 68, 104-109.

93            NESPOLO R.F, FIGUEROA J,SOLANO-IGUARAN J.J. (2017). Studying the evolutionary signicance of thermal adaptation in ectgotherms: The diversification of amphibians energetics. Journal of Thermal Biology, 68, 5-13.

92            MALDONADO K., SABAT T, PÍRIZ G, BOGDANOVICH J.M, NESPOLO R.F, BOZINOVIC F. (2017). Is maximun food instake in endotherms contrained by net or factorial aerobic scope? Lessons from the Leaf-Eared mouse. Frontiers in Physiology. 7: 649.

91            NESPOLO R.F., SOLANO-IGUARAN J.J, &F BOZINOVIC (2017).Phylogenetic Analysis Supports the Aerobic-Capacity Model for the Evolution of Endothermy. The American Naturalist. 189: 1, 13-27

90            FRANCO M, CONTRERAS C, PLACE N, BOZINOVIC F & NESPOLO R.F.(2017).Leptin levels, seasonality and thermal acclimation in the Microbiotherid marsupial Dromiciops gliroides: Does photoperiod play a role?. Comparative Biochemistry and Physiology, Part A. 233–240

89            PALEO-LOPEZ R, QUINTERO-GALVIS J.F, SOLANO-IGUARAN J.J, SÁNCHEZ-SALAZAR, A.M, GAITÁN-ESPITIA J.D & NESPOLO R.F.(2016). A phylogenetic analysis of macroevolutionary patterns in fermentative yeasts. Ecology and Evolution. 6,3851-3861.

88            HADJ-MOUSSA, H., MOGGRIDGE, J.A., LUU, B.E., QUINTERO-GALVIS, J.F., GAITÁN-ESPITIA, J.D., NESPOLO, R.F. & STOREY, K.B. (2016) The hibernating South American marsupial, Dromiciops gliroides,displays torpor-sensitive microRNA expression patterns. Scientific Reports. 6, 24627.

87            NESPOLO RF, SILVA AX, FIGUEROA F, & BACIGALUPE LD. (2015). Anticipatory gene regulation driven by maternal effects in an insect–host system. Ecology and Evolution. 5:23, 5601-5608

86            BARTHELD JS, GAITÁN-ESPITIA JD, ARTACHOP, SALGADO?LUARTE C, GIANOLIE & NESPOLO RF(2015). Energy expenditure and body size are targets of natural selection across a wide geographic range, in a terrestrial invertebrate. Functional Ecology. 29:1, 1463-1474

85            GAITÁN-ESPITIA JD & NESPOLO RF(2014). Is there metabolic cold adaptation in terrestrial ectotherms? Exploring latitudinal compensation in the invasive snail Cornu aspersum. The Journal of experimental biology. 217:13, 2261-2267

84            CORTÉS P, FRANCO M, MORENO-GÓMEZ F, BARRIENTOS K & NESPOLO RF(2014) Thermoregulatory capacities and torpor in the South American marsupial, Dromiciops gliroides. Journal of thermal biology 45, 1-8

83            NESPOLO RF& ROFF DA (2014) Model Falsification, Quantitative Genetics, and the Evolution of Endothermy: Are We Choosing the Right Tool? (A Reply to Hayes). American Naturalist 184: 6, 804-805

82            CONTRERAS C, FRANCO M, PLACE N & NESPOLO RF(2014). The effects of poly-unsaturated fatty acids on the physiology of hibernation in a South American marsupial, Dromiciops gliroides. Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology 177, 62-69

81            NESPOLO RF,BARTHELD JL, GONZALEZ A, BRUNING, A, ROFF DA, BACIGALUPE LD & GAITÁN-ESPITIA JD, (2014) The quantitative genetics of physiological and morphological traits in an invasive terrestrial snail: additive vs. non-additive genetic variation. Functional Ecology 28:3, 682-692

80            NESPOLO RF& ROFF DA (2014) Testing the aerobic model for the evolution of endothermy: implications of using present correlations to infer past evolution. American Naturalist 183: 1, 74-83.

79            FRANCO M., CONTRERAS C. CORTÉS P., NESPOLO RF.(2013). Aerobic power, huddling and the efficiency of torpor in the South American marsupial, Dromiciops gliroides. Integrative and Comparative Biology. 53: 1, E284-E284

78            GAITÁN-ESPITIA JD, ARIAS MB, LARDIES MA, NESPOLO RF (2013) Variation in thermal sensitivity and thermal tolerances in an invasive species across a climatic gradient: lessons from the land snail Cornu aspersum. PLOS ONE e70662.

77            NESPOLO RF, SCHEIHING† RA & ARTACHO P (2013) Sexual selection on locomotor performance in the calanoid copepod Tigriopus californicus. Evolutionary Ecology Research 15: 557–566.

76            BACIGALUPE LD, BARRIENTOS K, BECKERMAN AP, CARTER M, FIGUEROA CC, FOSTER SP, MOORE AJ, SILVA AX & NESPOLO RF(2013) Can invasions occur without change? A comparison of G matrices and selection in the peach-potato aphid, Myzus persicae. Ecology & Evolution 3:15, 5109-5118.

75            FRANCO M, CONTRERAS C & NESPOLO RF (2013) Profound changes in blood parameters during torpor in a South American marsupial. Comparative Biochemistry and Physiology A -Molecular & Integrative Physiology. 166:2, 338-342

74            BRUNING A, GONZALEZ A, GAITAN-ESPITIA JD, BARTHELD, JL & NESPOLO RF(2013) Metabolism, growth and the energetic definition of fitness: A quantitative genetic study in the land snail Cornu aspersum. Physiological and Biochemical Zoology 86: 538-546.

73            BRUNING A, GONZALEZ A, GAITAN-ESPITIA JD, BARTHELD, JL, TOADER-WILLIAMS, A, MONDACA F, NESPOLO RF(2013) Energy metabolism, heart rate and physiological differentiation in the pulmonate gastropod cornu aspersum. Journal of Molluscan Studies 79: 257-262.

72            GAITÁN-ESPITIA JD, SCHEIHING R, POULIN E, ARTACHO P & NESPOLO RF(2013) Mitochondrial phylogeography of the land snail Cornu aspersum: tracing population history and the impact of human-mediated invasion in austral South America. Evolutionary Ecology Research 15: 61-78.

71            GAITÁN-ESPITIA, BRUNING A, MONDACA F & NESPOLO RF(2013) Intraspecific variation in the metabolic scaling exponent in ectotherms: Testing the effect of latitudinal cline, ontogeny and transgenerational change in the land snail Cornu aspersum. Comparative and Biochemistry and Physiology A 165:169-177.

70            GAITÁN-ESPITIA, NESPOLO RF& OPAZO JC (2013) The complete mitochondrial genome of the land snail Cornu aspersum (Helicidae: Mollusca): intra-specific divergence of protein-coding genes and phylogenetic considerations within Euthyneura. PLOS ONE e67299.

69            DLUGOSZ EM, CHAPPELL MA, MEEK TH, SZAFRA?SKA P, KAROL Z, KONARZEWSKI M, JONES JJ, BICUDO E, NESPOLO RF, CAREAU V, AND  GARLAND T (2013). Phylogenetic analysis of mammalian maximal oxygen consumption during exercise. Journal of Experimental Biology. 216: 24, 5109-5118

68            CASTAÑEDA LD & NESPOLO, RF(2013) Phenotypic and Genetic Effects of Contrasting Ethanol Environments on Physiological and Developmental Traits in Drosophila melanogaster. PLOS ONE 8:e58920

67            WITHERS PC, COOPER CE, NESPOLO RF(2012) Evaporative water loss, relative water economy and evaporative partitioning of a heterothermic marsupial, the Monito del Monte (Dromiciops gliroides). Journal of Experimental Biology. 215, 2806-2813.

66            GAITÁN-ESPITIA J, FRANCO M, BARTHELD JL, NESPOLORF (2012) Repeatability of energy metabolism and resistance to dehydration in the invasive slug Limax maximus. Invertebrate Biology 131: 11-18.

65            CARTER M, SIMON JC, NESPOLORF (2012). The effects of reproductive specialization on energy costs and fitness genetic variances in cyclical and obligate parthenogenetic aphids. ECOLOGY AND EVOLUTION DOI: 10.1002/ece3.247

64            URREJOLA S, NESPOLORF, LARDIES MA (2011). Diet-induced  developmental plasticity in life-histories and energy metabolism in a beetle. Revista Chilena de Historia Natural 84: 523-533.

63            NAYA DE, CATALAN T, ARTACHO P, GAITÁN-ESPITIA JD, NESPOLORF (2011) Exploring the functional association between physiological plasticity, climatic variability, and geographical latitude: lessons from land snails. Evolutionary Ecology Research 13:647-659.

62            ARTACHO P, FIGUEROA CC, CORTÉS C, SIMON JC, NESPOLORF (2011) Short-term consequences of reproductive mode variation on the genetic architecture of energy metabolism and life-history traits in the pea aphid
Journal of Insect Physiology 57: 986-994.

61            CORTÉS P, FRANCO M, SABAT P, QUIJANO SA, NESPOLORF (2011) Bioenergetics and intestinal phenotypic flexibility in the marsupial, Monito del Monte (Dromiciops gliroides) from the temperate forest in South America. Comparative Biochemistry and Physiology A 160: 117-124.

60            NESPOLORF, CORREA L, PEREZ-APABLAZA CX, CORTÉS P, BARTHELD JL (2011) Energy metabolism and the postprandial response of the Chilean tarantulas, Euathlus truculentus(Araneae: Theraphosidae). Comparative Biochemistry and Physiology A 159: 379-382 .

59            NESPOLORF, BACIGALUPE LD, FIGUEROA CC, KOTEJA P, OPAZO JC (2011)    Using new tools to solve an old problem: the evolution of endothermy in vertebrates. Trends in Ecology and Evolution 26: 414- 423.

58            SCHEIHING  R, CARDENAS LC, LABARCA P,  NESPOLORF (2011). Viability selection on body size in a non-marine ostracod.  Hydrobiologia 671: 193-203.

57            SCHEIHING  R, CARDENAS LC, LABARCA P,  NESPOLORF (2011). Spatial and temporal analysis of the genetic diversity and population structure in a freshwater ostracod from the high Andean plateau. Evolutionary Ecology Research 13: 91-104.

56            NESPOLORF, RD SEPULVEDA, LD CASTAÑEDA, D ROFF (2011) Effects of shape variations on the energy metabolism of the sand cricket Gryllus firmus: a geometric morphometrics analysis. Biological Research 44: 69-74.

55            NESPOLORF (2010) Is the evolutionary theory still useful? A review with examples. Revista Chilena de Historia Natural 83: 479-495.

54            CASTAÑEDA LE, FIGUEROA CC, BACIGALUPE LD & NESPOLORF (2010) Effects of wing polyphenism, aphid genotype and host plant chemistry on energy metabolism of the grain aphid, Sitobion avenae. Journal of Insect Physiology 56: 1920-1924.

53            CASTAÑEDA LE, FIGUEROA CC, & NESPOLORF (2010) Do insect pests perform better on highly defended plants? Costs and benefits of induced detoxification defences in the aphid Sitobion avenae. Journal of Evolutionary Biology 23: 2474-2483.

52            SCHEIHING  R, LABARCA P SANTIBAÑEZ P, ASENCIO G, NESPOLORF (2010). A quantitative survey of the aquatic invertebrate community in the «Monumento Natural Salr de Surire» from Chilean altiplano. Journal of Natural History 637: 45-52.

51            CASTAÑEDA LE, FIGUEROA CC, & NESPOLORF (2010) Physiological approach to explain the ecological success of «superclones» in aphids: interplay between detoxification enzymes, metabolism and fitness. Journal of Insect Physiology 56: 1058-1064.

50            NESPOLORF, CORTÉS P, VERDUGO C, BACIGALUPE LD (2010) Bioenergetics of torpor in the Microbiotherid marsupial, Monito del Monte (Dromiciops gliroides): the role of temperature and food availability. Journal of Comparative Physiology B180: 767-773.

49            SCHEIHING  R, CARDENAS L, NESPOLORF, KRALL P, WALZ K, SHIRO KOHSHIMA, LABARCA P (2010) Morphological and Molecular analysis of South American Centropagidae family (Copepoda: Calanoidea). Hydrobiologia 637: 45-52.

48            BACIGALUPE LD, BUSTAMANTE D, BOZINOVIC F, NESPOLORF. (2010) Phenotypic integration of morphology and energetic performance under routine capacities: a study in the leaf-eared mouse Phyllotis darwini. Journal of Comparative Physiology B160:293-299.

47            NESPOLORF, HALKETT F, FIGUEROA CC, PLANTEGENEST, M & SIMON JC (2009) Evolution of trade-offs between sexual and asexual phases and the role of reproductive plasticity in the genetic architecture of aphid life histories. Evolution 63: 2402-2412.

46            NESPOLORF & BACIGALUPE LD (2009) Viability selection on early body mass and the effect of female body size on fecundity: a study on the leaf-eared mouse Phyllotis darwini. Ecological Research 24: 997-1002.

45            ARTACHO P & NESPOLORF (2009) Intrapopulation variation in the standard metabolism of a terrestrial mollusc: repeatability of the CO2 production in the land snail Helix aspersa. Physiological and Biochemical Zoology 82: 181-189.

44            CORTES P, QUIJANO SA & NESPOLORF (2009)  Bioenergetics and inter-individual variation in physiological capacities in a relict mammal, the monito del monte (Dromiciops gliroides). Journal of Experimental Biology 212: 297-207.

43            CASTAÑEDA LE, FIGUEROA CC, FUENTES E, NIEMEYER H & NESPOLORF (2009) Energy costs of detoxification systems in aphids feeding on chemically defended host-plants: a correlational study in the grain aphid, Sitobion avenae. Journal of Experimental Biology 212: 1185-1190.

42            ARTACHO P & NESPOLORF (2009) Natural Selection on Reducing Energy Metabolism in the Garden Snail (Helix aspersa). Evolution 63: 1044-1050.

41            NESPOLORF, D FAIRBAIRN & DA ROFF (2008). Energetic trade-off between maintenance costs and dispersion capacity in the sand cricket (Gryllus firmus). Functional Ecology 22: 624-632.

40            NESPOLORF,  PARTACHO, LE CASTAÑEDA, C VERDUGO (2008) Short-term thermoregulatory adjustments in a South American anseriform, the black-necked swan (Cygnus melanocoryphus). Comparative Biochemistry and Physiology A 150: 366-368.

39            NESPOLORF, CC FIGUEROA, M PLANTEGENEST, JC SIMON (2008) Short-term population differences in the genetic architecture of life history traits related to sexuality in an aphid species. Heredity 100: 374-381.

38            ARTACHO, P, LE CASTAÑEDA, M SOTO-GAMBOA, C VERDUGO & RF NESPOLO(2007) Using haematological parameters to assess the health and nutritional status of an endangered Black-Necked swan population. Comparative Biochemistry and Physiology A147: 1060-1066.

37            NESPOLO, RF & M FRANCO (2007) Metabolic rate is a repeatable trait: a meta analysis. Journal of Experimental Biology 210:  2000-2005.

36            ALCAPAN, AC, RF NESPOLO& JE TORO (2007) Heritability of body size in the Chilean blue mussel (Mytilus chilensis Hupé 1854): effects of environment and aging. Aquaculture Research 38: 313-320.

35            NESPOLO, RF, P ARTACHO & LD CASTAÑEDA (2007) Cyclic gas-exchange in the Chilean red cricket: inter-individual variation and thermal dependence. Journal of Experimental Biology 210: 668-675.

34            NESPOLORF (2007) A complex population dynamic explained by a single-l       ocus Mendelian model for litter size. Heredity 98: 63-64.

33            NESPOLO, RF, LE CASTAÑEDA & DA ROFF (2007). Quantitative genetic variation of metabolism in the nymphs of the sand cricket, Gryllus firmus, inferred from an analysis of inbred-lines. Biological Research 40: 5-12.

32            ARTACHO, P, LE CASTAÑEDA, M SOTO-GAMBOA, C VERDUGO & RF NESPOLO(2007). Blood biochemistry in a population of black-necked swans (Cygnus melanocoryphus) in a conservation priority area. Comparative Biochemistry and Physiology A146: 283-290.

31            CASTAÑEDA LE P SABAT SP GONZALES & RF NESPOLO(2006). Digestive plasticity in tadpoles of the Chilean giant frog (Caudiverbera caudiverbera): factorial effects of diet and temperature. Physiological and Biochemical Zoology 79: 919-926.

30            NESPOLORF, LE CASTAÑEDA & DA ROFF (2005). Dissecting the variance-covariance structure in insect physiology: the multivariate association between metabolism and morphology in the nymphs of the sand cricket (Gryllus firmus). Journal of Insect Physiology 51: 913-921.

29            NESPOLO, RF, LD BACIGALUPE, DM BUSTAMANTE & F BOZINOVIC (2005). Quantitative genetics of bioenergetics and growth-related traits in the wild mammal, Phyllotis darwini. Evolution 59: 1829-1837.

28            NESPOLORF (2005). New invariants and dimensionless numbers: futile renaissance of old fallacies? Biological Research 38: 27-29.

27            NESPOLORF & P ARTACHO (2005). How general are current comparative physiology studies? A quantitative review. Revista Chilena de Historia Natural 78: 313-321.

26            ARTACHO P, LE CASTAÑEDA & RF NESPOLO(2005). The role of quantitative genetic studies in animal physiological ecology. Revista Chilena de Historia Natural 78: 161-167.

25            BACIGALUPE LD, RF NESPOLO, D BUSTAMANTE & F BOZINOVIC (2005). Is there a trade-off betweeen energetics and spleen mass? A quantitative genetic study in the leaf-eared mouse. Evolutionary Ecology Research 7: 1-7.

24            NESPOLORF, LE CASTAÑEDA & DA ROFF (2005). The effect of fasting on activity and resting metabolism in the sand cricket, Gryllus firmus: a multivariate approach. Journal of Insect Physiology 51: 61-66.

23            BACIGALUPE LD, RF NESPOLO, JC OPAZO & F BOZINOVIC (2004). The role of thermal history on phenotypic plasticity in thermogenic capacity and organ size in the leaf-eared mouse Phyllotis darwini. Physiological and Biochemical Zoology 77: 805-815.

22            SABAT P, RF NESPOLO& F BOZINOVIC (2004). Water economy of three Cinclodes (Furnariidae) species inhabiting marine and freshwater ecosystems. Revista Chilena de Historia Natural 77: 219 – 225.

21            CARTER, MJ, MA LARDIES, RF NESPOLO& F BOZINOVIC (2004). Heritability of progeny size in a terrestrial isopod: transgenerational environmental effects on a life history trait. Heredity 93: 455 – 459.

20            BACIGALUPE LD, RF NESPOLO, D BUSTAMANTE & F BOZINOVIC (2004). The quantitative genetics of sustained energy budget in a wild mouse. Evolution 58: 421-429.

19            NESPOLO, RF (2003). Evolution by natural selection: more evidence than ever before. Revista Chilena de Historia Natural 76:699-716.

18            NESPOLO, RF, MA LARDIES & F BOZINOVIC (2003). Intrapopulation variation in the standard metabolic rate of insects: repeatability, thermal dependence and sensitivity (Q10) of oxygen consumption in a cricket. Journal of Experimental Biology 206: 4309-4315.

17            NESPOLO, RF, LD BACIGALUPE & F BOZINOVIC (2003). Heritability of energetics in a wild mammal, the leaf-eared mouse (Phyllotis darwini). Evolution 57: 1679-1688.

16            NESPOLO, RF, M ARIM & F BOZINOVIC (2003). Body size as a latent variable in a structural equation model: thermal acclimation and energetics of the leaf-eared mouse. Journal of Experimental Biology 206: 2145-2157.

15            REZENDE, EL, A CORTÉS, LD BACIGALUPE, RF NESPOLO& F BOZINOVIC (2003). Ambient temperature limits daily activity of the fossorial rodent Spalacopus cyanus. Journal of Arid Environments 55: 63-74.

14            NESPOLO, RF, LD BACIGALUPE & F BOZINOVIC (2003). The influence of heat increment of feeding on metabolic rate in Phyllotis darwini (Muridae). Comparative Biochemistry and Physiology A134: 139-145.

13            KENAGY, GJ, RA VASQUEZ, RF NESPOLO& F BOZINOVIC (2002). Daily and seasonal limits of time and temperature on surface activity of degus. Revista Chilena de Historia Natural. 75: 567-581.

12            BUSTAMANTE, DM, RF NESPOLO, EL REZENDE & F BOZINOVIC (2002). Dynamic thermal balance in small mammals: the interplay among ambient temperature, body size and behavior. Physiological and Biochemical Zoology 75: 396-404.

11            NESPOLO, RF, LD BACIGALUPE, P SABAT & F BOZINOVIC (2002). Interplay between energy metabolism, organ masses and digestive enzyme activity in the mouse-opossum, Thylamys elegans: the role of thermal acclimation. Journal of Experimental Biology 205: 2697-2703.

10            NESPOLO, RF & M ROSENMANN (2002).  Intraspecific allometry of hematological parameters in Basilichthys australis. Journal of Fish Biology 60: 1358-1362.

9              KENAGY, GJ, RA VASQUEZ, RF NESPOLO& F BOZINOVIC (2002). A time-energy analysis of summer daytime surface activity in degus, Octodon degus. Revista Chilena de Historia Natural 75: 149-156.

8              NESPOLO, RF, JC OPAZO & F BOZINOVIC (2001). Thermal acclimation and nonshivering thermogenesis in three species of South American rodents: a comparison between arid and mesic habitats. Journal of Arid Environments 48: 581-590.

7              NESPOLO, RF, LD BACIGALUPE, EL REZENDE, F BOZINOVIC (2001) When nonshivering thermogenesis equals maximum metabolic rate: thermal acclimation and phenotypic plasticity of fossorial cururos (Spalacopus cyanus, Rodentia). Physiological and Biochemical Zoology 74: 325-332.

6              NESPOLO, RF (2000) Desempeño fisiológico, estacionalidad y plasticidad fenotípica en pequeños mamíferos: microevolución de la capacidad de cambio en rasgos termorregulatorios. Revista Chilena de Historia Natural 73: 553 – 563.

5              OPAZO, JC, RF NESPOLO& F BOZINOVIC (1999) Arousal from torpor in the chilean mouse-opposum (Thylamys elegans): Does non-shivering thermogenesis play a role? Comparative Biochemistry and Physiology A 123: 399 – 408.

4              NESPOLORF J OPAZO, M ROSENMANN & F BOZINOVIC (1999) Thermal acclimation, maximum metabolic rate and nonshivering thermogenesis in Phyllotis xanthopygus (Rodentia) inhabiting the andean range. Journal of Mammalogy 80: 742 – 748.

3              NESPOLORF & M ROSENMANN (1997) Historia térmica de roedores: una aproximación experimental. Revista Chilena de Historia Natural  70: 363 – 370.

2              BOZINOVIC F &  RF NESPOLO(1997) Effect of ambient temperature and energy demands on digestive functions in leaf-eared mice (Phyllotis darwini) from central Chile. International  Journal of  Biometeorology 41: 23 – 25.

1              BOZINOVIC F, FF NOVOA & R NESPOLO(1997) Effect of dietary composition on food selection and assimilation in the leaf – eared mouse (Phillotys darwini) inhabiting central Chile. Revista Chilena de Historia Natural  70: 289 – 295.

 

BOOK CHAPTERS AND NON-ISI PUBLICATIONS

1. SOTO-GAMBOA M & RF NESPOLO(1997) Desarrollo de la homeotermia en dos aves antárticas: el efecto de la deprivación de ingesta. Serie Científica INACH 47: 31 – 37.
2. NESPOLO, RF.(2003)Termorregulación, vicuñas y esquila: un análisis exploratorio desde la fisiología comparada. En “Plan Nacional de Conservación y Manejo de la Vicuña (Vicugna vicugnaMolina, 1782) en Chile” (Galaz, JL & Gonzáles, G Eds.) Pp 43-54, OGRAMA SA. Santiago, Chile.
3. NESPOLO, RF.(2003) Genética cuantitativa y microevolución the caracteres fisiológicos. En: “Fisiología Ecológica de plantas y animales en Sudamérica: estudios interdisciplinarios” (F. Bozinovic Ed.). Pp. 27-42. Ediciones Universidad Católica de Chile. Santiago, Chile.
4. GUERRA, J., GAYOSO, J., SCHLATTER, J., NESPOLO, RF.(2005) Análisis de la biomasa de raíces en diferentes tipos de bosques. Avances en la evaluación de Pinus radiata en Chile. Bosque 26: 5-21.
5. SOTO-GAMBOA M, LAGOS N, QUIROZE, JARAMILLOE,NESPOLORF, CASANOVA-KATNYA (2007). On the causes of the disappearance of aquatic plant Egeria densa and black-necked swans in a Ramsar sanctuary: inappropriate statistical analyses, reduced scope of experimental design and the implausible chemical mechanisms proposed by Mulsow & Grandjean (2006). Ethics in Science and Environmental Politics 2007: 7-10.
6. NESPOLO, R.F., SÁENZ-AGUDELO, P., MEJÍAS, C., QUINTERO-GALVIS, J.F., PEÑA, I., SABAT, P., SÁNCHEZ-HERNÁNDEZ, J.C., GUROVICH, Y. (2022).Chapter 5: The Physiological Ecology of the Enigmatic Colocolo Opossum, the Monito del Monte (genus Dromiciops), and Its Role as a Bioindicator of the Broadleaf Biome, in: Larramendy, M.L., Liwszyc, G. (Eds.), Marsupial and Placental Mammal Species in Environmental Risk Assessment Strategies. Royal Society of Chemistry. pp. 81–111. https://doi.org/10.1039/9781839163470-00081